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The influence of solubility and adsorption on plastic materials on transport experiments
Šilhanová, Marie ; Kučera, Radim (advisor) ; Váňová, Nela (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department: Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Student: Marie Šilhanová Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Title of diploma thesis: The influence of solubility and adsorption on plastic materials on transport experiments From transport experiments on cell culture models we get valuable information about transport mechanism of drugs in organism. In vitro experiments are conducted for example on Transwell type inserts. During the experiment it was discovered that the results are not homogeneous, and the quantity of a substance in the solution decreases apparently, the reason behind this is inadequate solubility of lipophilic substances or their adsorption on the surface of plastic materials used in the experiment. Due to these problems we experience significant bias. This thesis is focused on antivirotics that did not perform well during transport experiments. First, HPLC/MS methods were developed, and they were used for concentration measurement of samples containing individual antivirotics. The drugs were tested under wide range of conditions so possible changes in effects of adsorption on plastic surfaces and solubility of drugs could be observed. The substances were divided into groups based on...
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DNA/RNA intercalating agents as antivirotics: possible mechanisms of action
Kropáček, Václav ; Španielová, Hana (advisor) ; Šmahelová, Jana (referee)
DNA/RNA intercalating agents are compounds with capability to insert themself between nucleic acids base pairs. This phenomenon is accompanied by structural or functional disruption of said nucleic acid. Some of these compounds are used as therapeutics for cancer, bacterial or parasital infection or are used as antivirotics. This work summarizes different mechanisms which are responsible for antiviral effects of three significant classes of intercalating agents, acridine derivates, quinolines and quinolones. Except for intercalation into nucleic acids, these compounds are also capable of alkalization of cellular organels, inhibition of some viral enzymes (helicase, integrase) and immunomodulation. These abilities enables them to disrupt viral life cycle.
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The influence of solubility and adsorption on plastic materials on transport experiments
Šilhanová, Marie ; Kučera, Radim (advisor) ; Váňová, Nela (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department: Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Student: Marie Šilhanová Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Title of diploma thesis: The influence of solubility and adsorption on plastic materials on transport experiments From transport experiments on cell culture models we get valuable information about transport mechanism of drugs in organism. In vitro experiments are conducted for example on Transwell type inserts. During the experiment it was discovered that the results are not homogeneous, and the quantity of a substance in the solution decreases apparently, the reason behind this is inadequate solubility of lipophilic substances or their adsorption on the surface of plastic materials used in the experiment. Due to these problems we experience significant bias. This thesis is focused on antivirotics that did not perform well during transport experiments. First, HPLC/MS methods were developed, and they were used for concentration measurement of samples containing individual antivirotics. The drugs were tested under wide range of conditions so possible changes in effects of adsorption on plastic surfaces and solubility of drugs could be observed. The substances were divided into groups based on...
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Preparation of the pandemic plan - the course of the influenza pandemy caused by the Pandemic A (H1N1) 2009 virus in the Pilsen region.
VELKOBORSKÁ, Marcela
An influenza is an illness annually affecting 5-15 percent of the world population. During the influenza pandemy 40-50 percent of world population can be affected and millions of people can die.The measures resulting from the pandemic plans help to limit the influenza virus spreading, to reduce morbidity and mortality. In April 2009 the first cases of the flue pandemic caused by Pandemic A (H1N1) 2009 virus occurred on the American continent, in the Czech Republic there was the first case registered in May, in the Pilsen region in July. Based on these facts I decided to assess the pandemic plans at the level of the Pilsen region and to analyse the course of the pandemy in the Pilsen region too. Having studied the Pandemic plan of the Pilsen region and the Pandemic plan of the Regional Hygiene Station of the Pilsen Region I got to the conclusion that in case of the pandemy caused by the highly virulent tribe of the influenza it would not be possible to use up the pandemic plans efficiently. The disadvantages concern mainly the way of the distribution of the pandemic vaccine and antivirotics. The other disadvantage is the absence of a parenteral form of antivirotics. A bad awarness of the inhabitants also came out effecting mass rejection of vaccination by the pandemic vaccine and preventative taking antivirotics. The analyse of the course of the pandemy in the Pilsen region proved that at many patients with the flue pandemic there was present a risky factor of more serious course of the influenza in the anamnesis. If these patients had been vaccinated by the pandemic vaccine they had been entitled for, they could have been protected against this illness, for some of them the vaccination might have meant life-saving. It was also proved that originally the pandemic tribe of Pandemic A (H1N1) 2009 virus became the causer of the common seasonal influenza in the season of 2010-2011.
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